室内不接触农药的褐飞虱种群对农药的敏感性和对水稻品种的适应性

doi:10.3969/j.issn.1001-7216.2013.04.012

中国水稻科学 ›› 2013, Vol. 27 ›› Issue (4): 419-424.DOI: 10.3969/j.issn.1001-7216.2013.04.012

室内不接触农药的褐飞虱种群对农药的敏感性和对水稻品种的适应性

董必琴^1,2 ,杨亚军^2,* ,徐红星² ,郑许松² ,K L HEONG³ ,吕仲贤^2,*

¹浙江师范大学化学与生命科学学院，浙江金华 321004；²浙江省农业科学院植物保护与微生物研究所，浙江杭州 310021；³国际水稻研究所，菲律宾马尼拉DAPO BOX 777；

收稿日期:2013-02-02 修回日期:2013-04-02 出版日期:2013-07-10 发布日期:2013-07-10
通讯作者: 杨亚军2,* ,吕仲贤2,*
基金资助:
国家十二五科技支撑计划资助项目（2012BAD19D03）；亚洲发展银行（ADB）区域性支撑项目（RETA6489）。

Susceptibility to Insecticides and Ecological Fitness on Resistant Rice Varieties of Population of Nilaparvata lugens Stl (Hemiptera: Delphacidae) without Exposure to Insecticide in Laboratory

DONG Biqin ^1,2, YANG Yajun ^2,* , XU Hongxing ², ZHENG Xusong ², K L HEONG ³ , LV Zhongxian ^2,*

¹ College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China; ²Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; ³International Rice Research Institute, DAPO BOX 777, Metro Manila, Philippines;

Received:2013-02-02 Revised:2013-04-02 Online:2013-07-10 Published:2013-07-10
Contact: YANG Yajun2,*, LV Zhongxian2,*

摘要/Abstract

摘要： 在室内研究了经连续多代未接触农药的田间褐飞虱种群在不同抗性水稻品种上的生态适应性。结果表明，田间种群经室内连续多代未接触农药饲养后其对吡虫啉的抗性从F1代的359.94倍降到F14代的6.50倍，其中F4到F8下降快，F10到F14代基本趋于稳定；而其对毒死蜱的抗性下降趋于平缓，从F1代的9.90倍降到F14代的5.94倍。若虫历期和雌成虫体质量均显著受水稻品种和连续饲养代别数及代别×品种的影响，而若虫存活率只受代别数影响。雄虫中短翅型比例受代别和代别×品种的影响，而雌虫中短翅型比例不受代别、品种以及代别×品种的影响。在三种水稻品种上褐飞虱若虫历期均随着代别增加而延长，其中F1和F4的若虫历期较短，其余均较长。在感虫水稻品种TN1上的雌虫若虫历期明显短于在抗虫品种IR26和IR36上的历期，TN1上的雌成虫体质量明显比抗虫品种上的高。这些结果表明褐飞虱种群不接触农药后其对农药的敏感性上升，但对水稻抗性品种IR26和IR36的适应性并未增强。这些结果对于合理使用农药和水稻抗性品种治理褐飞虱具有重要意义。

关键词: 褐飞虱, 农药, 生态适应性, 抗药性, 水稻品种

Abstract: Rice brown planthopper (BPH, Nilaparvata lugens) population collected from rice filed in Hangzhou was successively reared on susceptible rice TN1 in the laboratory without exposure to insecticides for more than 14 generations, and its susceptibility change to insecticides and ecological fitness on different resistant rice varieties were monitored by generations. The resistance to imidacloprid was sharply declined with increasing generations without exposure to insecticides from 359.94fold at F1 to 6.50fold at F14 compared with susceptible strain, and the resistance to chlorpyrifos was from 9.90fold at F1 to 5.94fold at F14. Nymphal duration, weight of newly hatched female adult was significantly affected by rice variety, generation and their interaction, meanwhile the nymphal survival was significantly affected by generations only. The ratio of brachypterous adults in male was affected by generations and generation × variety interaction, while no difference was found in female. Nymphal duration was prolonged with increasing generations, and female nymphal duration was shorter on susceptible variety TN1 than that on resistant varieties IR26 or IR36，meanwhile female adult weight on TN1 was higher than that on IR26 and IR36. These results indicated that resistance of field BPH population to insecticide was reversed after several generations without exposure to insecticide, and its ecological fitness on TN1 was better than that on IR26 and IR36. It implies an important role in BPH management by rational and reductive use of insecticides coordinating with manipulation of resistant rice cultivars.

Key words: Nilaparvata lugens, insecticide, ecological fitness, insecticidal resistance, rice variety

中图分类号:

S435.112+3

董必琴1,2 ,杨亚军2,* ,徐红星2 ,郑许松2 ,K L HEONG3 ,吕仲贤2,*. 室内不接触农药的褐飞虱种群对农药的敏感性和对水稻品种的适应性[J]. 中国水稻科学, 2013, 27(4): 419-424.

DONG Biqin1,2, YANG Yajun2,*, XU Hongxing2, ZHENG Xusong2, K L HEONG3, LV Zhongxian2,*. Susceptibility to Insecticides and Ecological Fitness on Resistant Rice Varieties of Population of Nilaparvata lugens Stl (Hemiptera: Delphacidae) without Exposure to Insecticide in Laboratory[J]. Chinese Journal of Rice Science, 2013, 27(4): 419-424.

参考文献

\[1\]程家安，朱金良，祝增荣，等. 稻田飞虱灾变与环境调控. 环境昆虫学报， 2008, 30(2): 167182.

\[2\]Catindig J, Arida G S, Baehaki S E, et al. Situation of planthoppers in Asia //Heong K L, Hardy B. Planthoppers: New Threats to the Sustainability of Intensive Rice Production Systems in Asia. Los Baos (Philippines): International Rice Research Institute， 2009: 221232.

\[3\]Heong K L. Are planthopper problems caused by a breakdown in ecosystem services//Heong K L, Hardy B. Planthoppers: New Threats to the Sustainability of Intensive Rice Production Systems in Asia. Los Baos(Philippines): International Rice Research Institute, 2009: 221232．

\[4\]Peng S, Tang Q, Zou Y. Current status and challenge of rice production in China. Plant Prod Sci, 2009, 12(1): 38.

\[5\]夏敬源. 我国重大农业生物灾害暴发现状与防控成效. 中国植保导报, 2008, 28(1): 59.

\[6\]姜辉，林荣华，刘亮，等. 稻飞虱的危害及再猖獗机制. 昆虫知识， 2005，42(6): 612615.

\[7\]Karban R, Chen Y. Induced resistance in rice against insects. Bull Entomol Res, 2007, 97(4): 327335．

\[8\]Panda N, Khush G S. Host Plant Resistance to Insects. CAB International, UK, 1995：67103．

\[9\]George W L, Heong K L, Johnson D, et al. Rice pest management: Issues and opportunities // Pandey S, Byerlee D, Dawe D, et al. Rice in the Globe Economy: Strategic Research and Policy Issues for Food Security. Los Baos (Philippines): International Rice Research Institute, 2010: 297332.

\[10\]Du B, Zhang W L, Liu B F, et al. Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice. PNAS, 2009, 106(52): 2216322168．

\[11\]Karban R, Chen Y. Induced resistance in rice against insects. Bull Entomol Res, 2007, 97(4): 327335.

\[12\]Medrano F G, Heinrichs E A. Response of resistant rice to brown planthopper collected in Mindanao, Philippines. IRRN, 1985, 10: 1415.

\[13\]吕仲贤, 俞晓平, 陶林勇, 等. 水稻新品种(系)对褐飞虱抗性的评价. 中国农业科学， 2002, 35(2): 225229.

\[14\]Singh J, Dhaliwal G S. Insect resistance in rice: Progress and perspectives//Dhaliwal G S, Singh R. Host Plant Resistance to Insect. New Delhi: Panima Publishing Corporation, 2004.

\[15\]陈峰, 傅强, 罗举, 等. 苗期抗性不同的水稻品种成株期对褐飞虱的抗性. 中国水稻科学, 2009, 23(2): 201206.

\[16\]Berrticat C, Boquien G, Raymond M, et al. Insecticide resistance genes induce a mating competition cost in Culex pipiens mosquitoes. Genet Res, 2002, 79(1): 4147.

\[17\]Boivin T, Bouvier J C, Chadoeuf J, et al. Constrains on adaptive mutations in the codling moth, Cydia pomonella (L.): Measuring fitness tradeoffs and natural selection. Heredity, 2003, 90(1): 107113.

\[18\]任晓霞, 韩召军, 王荫长, 等. 棉铃虫对久效磷抗性和敏感品系的生物适合度. 南京农业大学学报, 2001, 24(2): 4144.

\[19\]于金凤, 慕立义, 王开运，等. 抗性棉蚜生殖力和存活率的研究. 华东昆虫学报，1997，6(2): 5256.

\[20\]Arnaud L, Haubruge E. Insecticide resistance enhances male reproductive success in a beetle. Evolution, 2002, 56(12): 24352444.

\[21\]刘凤沂，须志平，薄仙萍, 等. 昆虫抗药性与适合度. 昆虫知识，2008，45(3): 374375.

\[22\]陈利, 陈建明, 何月平, 等. 抗感吡虫啉褐飞虱在抗虫水稻品种上的适合度比较. 应用昆虫学报, 2011, 48(5): 13811386.

\[23\]刘泽文, 韩召军, 王荫长. 褐飞虱抗有机磷品系的交互抗性和适合度研究. 南京农业大学学报, 2001, 24(4): 3740.

\[24\]Matsmura M，Takeuchi H, Satoh M，et al. Current status of insecticide resistance in rice planthoppers in Asia//Heong K L, Hardy B, Planthoppers: New Threats to the Sustainability of Intensive Rice Production Systems in Asia. Los Baos (Philippines): International Rice Research Institute， 2009： 233244.

\[25\]Liu Z W, Williamson M S, Lansdell S J, et al. A nicotinic acetylcholine receptor mutation conferring targetsite resistance to imidacloprid in Nilaparvata lugens (brown planthopper). PNAS, 2005, 102: 84208425.

\[26\]温玉丛，范凯华，师慈，等．褐飞虱对吡虫啉的田间抗性动态分析. 南京农业大学学报，2010，33(4):5558.

\[27\]张帅，李永平，邵振润. 2010年全国农业有害生物抗药性监测结果及科学用药建议. 中国植保导刊，2011, 31（4）:4041,16.

\[28\]杨普云, 熊延坤, 尹哲, 等. 绿色防控技术示范工作进展与展望. 中国植保导刊, 2010, 30(4): 3738.

\[29\]杨普云, 赵中华. 农作物病虫害绿色防控技术指南. 北京：中国农业出版社， 2012.

\[30\]刘泽文，刘成君，张洪伟, 等. 褐飞虱抗吡虫啉品系生物适合度研究.昆虫知识, 2003, 40(5): 419420.

\[31\]Wilson M, Moshitzky P, Laor E, et al.. Reversal of resistance to pyriproxyfen in the Q biotype of Bemisia tabaci (Hemiptera: Aleyrodidae). Pest Manag Sci, 2007, 63:761768.

\[32\]Keiding J. Possible reversal of resistance. Bull Wld Hlth Org, 1963, 29(Suppl): 5162.

\[33\]Liu Z W, Han Z J. Fitness costs of laboratoryselected imidacloprid resistance in the brown planthopper, Nilaparvata lugens (Stl). Pest Manag Sci, 2006, 62: 279282.

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室内不接触农药的褐飞虱种群对农药的敏感性和对水稻品种的适应性

Susceptibility to Insecticides and Ecological Fitness on Resistant Rice Varieties of Population of Nilaparvata lugens Stl (Hemiptera: Delphacidae) without Exposure to Insecticide in Laboratory

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